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A4503
Sigma
Albumin from bovine serum
Cohn V fraction, ≥96% (agarose gel electrophoresis), lyophilized powder
| Synonym: | Bovine albumin, BSA, Cohn Fraction V |
| CAS Number: | 9048-46-8 |
| EC Number: | 232-936-2 |
| MDL number: | MFCD00130384 |
Description
| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Preparation Note | Initial fractionation by cold alcohol precipitation Prepared by a modification of Cohn, using cold ethanol, pH, and low temperature precipitation. |
| Quality | Remainder mostly globulins. |
| Application | Bovine serum albumin (BSA) makes up approximately 50% of the total protein in serum. Although it may be used as a carrier for hapten immunization in animals other than goats and sheep, it is more commonly used as a carrier for hapten in immunoassays. The small size of BSA, its abundance in serum, and the similarity between BSA and albumins from other species makes it less desirable for antibody production. |
Properties
| assay | ≥96% (agarose gel electrophoresis) |
| form | lyophilized powder |
| mol wt | mol wt ~66 kDa |
| pH | 5.0-5.6 (1 % (w/v) in H2O) |
| EQP level | Premium |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
Related Products
| Comparable product | A2058, Albumin from bovine serum |
References
| reference | Cohn, E.J., et al. J. Am. Chem. Soc. 68, 459, (1946) |
| Trigatti, B.L., , Gerber G.E., A direct role of serum albumin in the cellular uptake of long-chain fatty acids. Biochem. J. 308 (Pt 1), 155, (1995) | |
| Merck | Merck 13,8542 |
